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中华普通外科学文献简介:中华普通外科学文献简介,中华普通外科学文献简介[详细]

期刊信息

主管:
中华人民共和国国家卫生健康委员会
主办:
中华医学会
承办:
中山大学附属第一医院
中山大学附属中山医院
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中华医学电子音像出版社有限责任公司
总编辑: 王深明
编辑部主任: 汪谦
刊 期: 双月刊
创刊时间: 2007年2月
国内刊号: 11-9148/R
国际刊号: 1674-0793
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标题: microRNA-301在肝细胞癌中的诊断价值研究
摘要:

目的:探讨microRNA-301检测在肝细胞癌中的诊断价值。方法:收集2014年1月至2015年7月中山市人民医院52例肝细胞癌手术患者术前血清标本(实验组),以及38名体检健康者血清标本作为对照组。定量PCR法测定两组患者血清microRNA-301基因表达水平,独立样本t检验分析表达差异;应用ROC曲线探索其诊断截断值,对microRNA-301表达水平进行诊断有效性评估;应用单因素分析患者microRNA-301表达水平与临床病理参数之间的关系;对microRNA-301相对表达水平和肝细胞癌巴塞罗那分期进行相关分析和方差分析。结果:(1)实验组患者血清microRNA-301相对表达水平为0.035±0.014,显著高于对照组的0.021±0.012,差异有统计学意义(t=4.793,P<0.001)。(2)ROC曲线最佳截断值为0.203 850,敏感度为86.5%,特异度为63.2%,ROC曲线下面积为0.77(P<0.01),诊断效力有一定准确性。(3)microRNA-301相对表达水平与患者年龄、性别及肿瘤大小无明显相关(P=0.409、0.236、0.096),与巴塞罗那分期、是否合并门静脉癌栓、甲胎蛋白(AFP)水平及HBsAg是否阳性相关(P=0.001、0.017、0.007、<0.001)。其中巴塞罗那分期对预后影响相对较大,Pearson相关系数为0.825(P<0.001)。(4)巴塞罗那分期早期、中期及晚期例数分别为7、25、20例,单因素方差分析显示早期和中期患者microRNA-301相对表达水平差异无统计学意义(P=0.545);晚期患者的microRNA-301相对表达水平显著高于早、中期患者(P=0.006、<0.001)。结论:microRNA-301作为一种癌基因microRNA,在肝细胞癌患者血清中表达上调,其相对表达水平与巴塞罗那分期、门静脉癌栓、AFP及HBsAg相关,对肝细胞癌具有一定的诊断价值。

英文摘要: Objective:To explore the diagnostic value of microRNA-301 in hepatocellular carcinoma. Methods:From January 2014 to July 2015, preoperative serum specimens of fifity-two patients with hepatocellular carcinoma in Zhongshan City People’s Hospital were collected as experimental group. Serum samples from 38 healthy subjects were collected as control. The level of microRNA-301 gene expression in two groups was measured by quantitative PCR and compared with independent sample t test. The ROC curve was used to explore the diagnostic truncation value and effectiveness. The relationship between the expression level of microRNA-301 and the clinicopathological parameters of hepatocellular carcinoma were preliminary analyzed. Correlation analysis and variance analysis were used to verify association of microRNA-301 level with Barcelona staging in hepatocellular carcinoma. Results:(1) The relative expression level of serum microRNA-301 in the experimental group was 0.035±0.014, which was significantly higher than that in the control group (0.021±0.012), the difference was statistically significant (t=4.793, P<0.001). (2) The best cut off value of ROC curve was 0.203 850, sensitivity was 86.5%, specificity was 63.2%, the area under ROC curve was 0.77 (P<0.01), and the diagnostic effectiveness was certain. (3) The relative expression level of microRNA-301 was not significantly correlated with age, sex and tumor size (P=0.409, 0.236, 0.096), while correlated with Barcelona stage, portal vein tumor thrombus, AFP level and HBsAg positivity (P=0.001, 0.017,0.007, <0.001). Barcelona staging had relatively large effect on prognosis, and the correlation coefficient of Pearson was 0.825 (P<0.001). (4) There were 7, 25 and 20 cases in the early, middle and late stages of Barcelonastaging, respectively. One-way ANOVA analysis showed that there was no significant difference in the relative expression level of microRNA-301 between the early and middle stages of patients (P=0.545), while the late stage was significantly higher than the early and middle stages (P=0.006, <0.001). Conclusions:microRNA-301, as an oncogene microRNA, is up-regulated in the serum of patients with hepatocellular carcinoma. The relative expression level of microRNA-301 is associated with Barcelona staging, portal vein tumor thrombus, AFP and HBsAg, and has certain diagnostic value for hepatocellular carcinoma.
作者:

何坤;胡泽民;阮嘉后;黄锐钦;罗启杰

作者单位: 中山,中山大学附属中山医院 中山市人民医院普外一科
期刊: 中华普通外科学文献(电子版)
年.卷(期):页码 2018 .12(5):314-318
中图分类号:
文章编号:
引用格式: [1]何坤;胡泽民;阮嘉后;黄锐钦;罗启杰.microRNA-301在肝细胞癌中的诊断价值研究[J/CD].中华普通外科学文献(电子版),2018,12(5):314-318 .
关键词: 癌 肝细胞 微RNAs microRNA-301 诊断价值
英文关键词: Carcinoma,hepatocellular MicroRNAs MicroRNA-301 Diagnostic value

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